Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a ) E. coli (ATCC 25922); ( b ) clinical isolate of imipenem-resistant E. coli with (red curves) and without (black curves) imipenem treatment; ( c ) signal ratio of 654-cm −1 SERS peak ( r 654 ) of E. coli (ATCC 25922) as a function of imipenem treatment time; ( d ) r 654 of imipenem-resistant E. coli as a function of imipenem treatment time; ( e ) signal ratio of 724-cm −1 SERS peak ( r 724 ) of E. coli (ATCC 25922) as a function of imipenem treatment time; ( f ) r 724 of imipenem-resistant E. coli as a function of imipenem treatment time. Black and red curves represent the mean SERS spectra, while gray and light red curves represent their corresponding standard deviation.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques: Standard Deviation

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: After being treated by antibiotics for 0, 0.5, 1 and 2 hours, the bacteria were stained with PI (fluorescing red) and SYTO 9 (fluorescing green). BF: bright-field image; FL: fluorescence image. ( a ) S. aureus (ATCC 29213) treated with oxacillin; ( b ) E. coli (ATCC 25922) treated with imipenem; ( c ) percentage of dead bacteria ( f dead ): S. aureus (black columns) and E. coli (gray columns). f dead = N r /( N g + N r − N g + r ), where N r is the count of the red signatures, N g is the count of the green signatures, and N g + r is the count that the green and red signatures overlap. The scale bars represent 20 μ m.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques: Bacteria, Staining, Fluorescence

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a ) Signal ratio of 730-cm −1 SERS peak ( r 730 ) of S. aureus versus vancomycin treatment time at concentrations of 0.5 μ g/ml (light gray columns), 1 μ g/ml (gray columns), and 2 μ g/ml (black columns); ( b , c ) signal ratios of 654-cm −1 SERS peak ( r 654 ) and 724-cm −1 SERS peak ( r 724 ) of E. coli , respectively, versus imipenem treatment time at concentrations of 0.03 μ g/ml (light gray columns), 0.06 μ g/ml (gray columns), and 0.12 μ g/ml (black columns).

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques:

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a – c ) Signal ratio of 654-cm −1 SERS peak ( r 654 ) of E. coli of inoculum densities of 10 6 , 10 7 and 10 8 CFU/ml, respectively, after being treated with different concentrations of imipenem for 2 hrs.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Structure of pantothenic acid and N-substituted pantothenamides

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Synthetic route to new N-substituted pantothenamides

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Antibacterial activity of N -substituted pantothenamides 9a–9m

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques: Activity Assay